Transformace zelené řasy \kur{Desmodesmus quadricauda}

The aim of this study was to investigate if electroporation can be used for a preparation of stable transformants of the green alga Desmodesmus quadricauda. I tested a sensitivity of D. quadricauda cells to several antibiotics in order to choose a suitable selection marker. I also tried to optimize electroporation conditions. I tested several electroporation methods and I tried transformation of cells of asynchronous cultures and in different stages of the cell cycle in synchronous culture. I tested the effect of sonication and glass beads on cell wall of coenobia in order to slightly damage it, release individual cells and, thus, affect the transformation efficiency