Monitoring of the pharmaceuticals and pesticides occurrence in the upper part of Blanice river using passive sampling approach

The goal of this thesis was to determine the occurrence of pharmaceuticals and pesticides at the upper part of Blanice river. The spring of the river is located in the Military area Boletice. In this area is minimal incidence of people and minimal land use. It was assumed minimum level of pollution at this part of the monitored area. Higher pollution level was supposed in the downstream part of the monitored area relating to higher population density and agriculture. POCIS passive samplers were used for collecting samples. Analysis were performed by liquid chromatography. There were 201 substances (127 pharmaceuticals, 74 pesticides). The concentrations of 27 substances (18 pharmaceuticals, 9 pesticides) were above the limit of quantification. The lowest level of pollution was found at Spalenec site with total pharmaceuticals and pesticides concentration 83.9 ng. POCIS-1. The highest level of contamination was found at the site Černý stream - 618.9 ng. POCIS-1. However this is only a small stream. The second highest contamination was found in the Rechle site close to the inlet to Husinec drinking water reservoir. Total level of pharmaceuticals and pesticides at this site was amounted to 482 ng. POCIS-1. In the upper part of the monitored catchment area is relatively low level of settelment and use of landscape. There is also low level of pharmaceuticals and pesticides pollution. At the Řepešín mill is forestry landscape changing to agricultural landscape and number of inhabitant is rising up. From this site pollution increases considerably in the landscape. This effect was then reflected in our results. Some substances in the environment change its chemical properties and become persistent. Therefore they are present in the landscape long time after their prohibition for using (e.g. hexazinone, DEET). The Husinec water reservoir reduces the amount of contaminants in the water of Blanice river. This effect was more pronounced for pharmaceuticals than pesticides