The effect of new synthetized drugs on electrical activity of rat isolated heart

This thesis deals with the influence of the newly synthesized drug on the electrical activity of the isolated heart of rat. Part of the thesis is a theoretical analysis of the use laboratory animals in experiments and ethical aspects related to the use of laboratory animals. There is also an analysis of drug testing, test substances, electrocardiography and methods used to detect and measure ECG signal. The two algorithms used for the QT interval are automated. The QT interval is the main indicator of cardiotoxicity and is considered to be the gold standard in evaluating the effect of the drug. In the practical part the ECG records obtained at the Faculty of Medicine at Masaryk University in Brno are processed. These records are dimmed manually and automatically. The manual dimming was consulted with a specialist in cardiography and statistically processed. Statistical processing served to compare with the results of the automatic ECG measurement. The algorithm is used to automate the measurement, and the results are compared with the reference points obtained from cardiology experts and manual measurement results. This work serves as a pilot study for the development and testing of a new active substance