Soluble forms of TLR receptors - a new biomarkers of histological chorioamnionitis in pregnancy complicated by preterm prelabor rupture of the membranes.

Background: Premature birth is a birth between 24th and 37th gestational weeks. Risk of preterm birth is in an increase of perinatal morbidity and mortality. Approximately in 3% of all pregnancies premature rupture of membranes (preterm premature rupture of membranes, PPROM) occurs. PPROM is responsible roughly for one-third of premature births. Among the important risk factors of PPROM infection is considered. The innate immunity has the decisive influence on the type and intensity of immune response to the microorganism present in the cavity, and is in particularly responsible for identifying the PAMP, pathogen-associated molecular patterns by specific receptors (PRR). The most important PRR are TLR receptors. They are primarily membrane expressed, although due to influence of many variable factors they can be shed from the membrane and can act in a soluble form. The aim of study our study is to measure the levels of soluble forms of PRR, especially sTLR2 sTLR4 and in amniotic fluid of patients with PPROM and to evaluate their possible diagnostic significance. Methods: The study included 44 pregnant women with gestational age between 24th and 36th weeks gestation, of whom 23 patients had histologically documented chorioamnionitis. From this group, 9 patients had particularly severe inflammation,..