Study of plasma protein binding of new radiopharmaceuticals

CHARLES UNIVERSITY IN PRAGUE FACULTY OF PHARMACY IN HRADEC KRÁLOVÉ Department of Pharmacology and Toxicology DIPLOMA THESIS: Study of plasma protein binding of new radiopharmaceuticals SUMMARY: The binding ability of two receptor specific peptides - 111 In - DOTA - minigastrin 45 and 177 Lu - DOTA - bombesin - was studied by methods of equilibrium dialysis at 37řC and ultrafiltration in terms of interspecies comparison. Among selected animal species (rat, rabbit, bovine, human) was plasma protein binding completely different. Higher binding was observed for 111 In - DOTA - minigastrin 45. In comparison of both methods - higher values of binding were measured for both peptides and all species by ultrafiltration than by equilibrium dialysis. KEYWORDS: radiopharmaceuticals, plasma protein binding, equilibrium dialysis, ultrafiltration, 111 In - DOTA - minigastrin 45, 177 Lu - DOTA - bombesin Barbora Šejnová Hradec Králové 201