Mechanismy regulace aktivity vynašečů auxinu

The morphology of plant body is shaped by genetically coded developmental programme together with environmental factors. The influence of environmental factors on the morphology is much more important in plants that in other multicellular organisms. The developmental programme provides a general scheme that is modified by environmental signals. Phytohormone auxin is a regulator of plant morphogenesis and its distribution in the plant body is an important mechanism controling the growth and development of plants and coordinating the developmental programme with environmentally-induced changes. This thesis investigates factors important for transport of auxin from cells, second part is dedicated to bioinformatic analysis of the transporters from the PIN protein family. For investigation of signals involved in regulation of activity of auxin efflux transporters I have selected (based on published information) physiological signals influencing auxin transport. These signals were changed by treatment with chemicals and resulting changes in auxin transport were measured (on the cellular level) with the intention to discover signals that can rapidly (within minutes) change the activity of auxin efflux transporters. The signals, which satisfy these requirements were subjected to further investigation. He detailed..