In vitro cultures of medicinal plants - XIII.

Jan Kubeš Genista tinctoria in vitro - abiotická elicitace Cultures of medicinal plants in vitro - XIII. The plants cultures in vitro contain lesser amounts of secondary metabolites in compare with intact plants. The elicitors can affect these metabolites production. The effect of electric current (50mA) of different voltage (5, 7, 8, 10, 15, 20 and 24 V) and different time exposition (10, 30, and 60 minutes) on content of isoflavones was studied on (Genista tinctoria) suspension culture. The highest concentration of genistin (0.17 %) was measured in suspension culture after 30 min of elicitation by 10 V after 6 hours of cultivation. The highest concentration of daidzein (0.35 %) was found in suspension culture after 60 min of elicitation by 5 V after 24 hours of cultivation. The highest concentration of genistin (1.6 mg/100ml) was measured in medium after 30 min of elicitation by 5 V after 24 hours of cultivation. The highest concentration of daidzein (1.77 mg/100ml) was found in medium after 10 min of elicitation by 24 V after 6 hours of cultivation