The Introduction of Immunohistochemistry in Laboratory Practice for Prostate Disease

The introduction of immunohistochemistry in laboratory practice for prostate disease Němečková Lenka ABSTRACT Increased incidence of benign prostatic hyperplasia (BPH) has been associated with age of men. The quality of lives gets worse in men with BPH. Higher numbers of reported malignant tumours of prostate with age of men, too. Adenocarcinoma of prostate (CaP) was the most frequently diagnosed oncological disease in males after other malignant neoplasms of skin. Physical examination and laboratory methods, especially the measurement of prostatic specific antigen (PSA) in serum, are applied in the diagnosis of CaP. The histological examination of tissue sample obtained by needle biopsy of the prostate confirms the diagnosis of CaP. The immunohistochemical examination of histological sections helps to differentiate malignant and benign prostatic glands in tissue of prostate usually using antibody anti - high molecular weight cytokeratins (HMW CK) highlighting basal cell of benign prostatic glands. Basal cell is missing in malignant glands. Method of immunohistochemistry was introduced and is used in the diagnosis of CaP in the histology laboratory at department of Pathology in Oblastní nemocnice Náchod (ONN). In the laboratory needle prostate biopsies are investigate using monoclonal antibody anti - HMW CK..