Development of Method for Determination of Endocrine Disruptors in Environmental Samples

There are many substances of natural and anthropogenic origin at present called endocrine disruptors which may influence endocrine system of animals and humans. A complex analysis of endocrine disruptors in environmental samples is important for knowledge of their behaviour in living environment and for their risk estimation. A complex method has been developed in this thesis for an analysis of bisphenol A, 17α-ethinylestradiol, irgasan and 4-nonylphenol in activated sludge and soil involving ASE extraction, sludge extract cleaning with silicagel and GPC and subsequent GC/MS and HPLC/UV samples quantification. These final extraction conditions of ASE have been settled: extraction mixture DCM:acetone (3:1 v/v), temperature 60 řC, pressure 1700 psi, 3 extraction cycles. ASE yield during soil extraction has been settled in range of 93,0-116,3 % with RSD 2,7-5,7% by HPLC/UV analysis and 93,3-106,0 % with RSD 9,3-15,6 % by GC/MS analysis. The whole method yield including the cleaning steps has been settled in range of 78,2-111,2 % with RSD 4,1-10,6 % in HPLC/UV quantification and 74,6-88,9 % with RSD 3,7-18,2 % in GC/MS quantification. The method application on the artificially contaminated sludge and soil samples analyzed after five months of storage has provided the yield of all analysts in range of..