Investigation of cytotoxicity in selected xenobiotics

The subject of the work was to evaluate the in vitro cytotoxicity of selected xenobiotics using a suitable cellular model. The aim was to determine a possible cytotoxic effect of potential antiinfective compounds from two different chemical groups. The first group includes representatives of the newly synthesized derivatives of substituted pyrazin-2,3-dikarbonitril (ZIP-34, ZIP- 128, ZIP-130 a ZIP-136) and the second group is made of derevatives of chitosan with bound antituberculotic active substances (Chi-2-SP and Chi-7). The tested samples were supplied by the Department of Organic and Inorganic Chemistry and Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague. To determine the cytotoxicity, a standard colorimetric method based on an evaluation of the metabolic state of cells was used. As the experimental model for assessment of hepatoxicity the standard human hepatic cell line HepG2 was selected. As the parameter for assessment of cytotoxicity of the tested compounds the values IC50 were measured. The values IC50 were identified for all substances from the group of ZIPs. The highest toxicity was found for ZIP-128 and the lowest for ZIP-136. But overall, the performed tests showed that the values IC50 are very similar and..