Bioactive substance of Rheum race used in Traditional Asian Medicine.

Vanda Neradiová Biologically active substances in the genus of Rheum species used in traditional Asian medicine Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Specialist in laboratory methods Background The goal of this study was to determine content substances and their quantity in two different species of genus Rheum and compare to what extent these two species differ in a number of content substances. Another criterion by which these species were compared was their place of origin. I have compared a species growing in its natural environment in the Himalayas with a second species growing in the Czech Republic. Methods For determination of content substances and their quantitative determination high - performance liquid chromatography with UV and fluorescent detection was used. Samples were compared with retention factors and retention times of standards. Furthermore, isolation and identification of probable physcion was carried out using mass spectrometry. Results By qualitative analysis of antraquinones, aloe - emodin, emodin, chrysophanol, physcion and rhein were determined and by analysis of stilbenes piceatannol and resveratrol were determined. Type R. acuminatum had greater representation of antharaquinons compared to R. australe. When comparing content..