Monitoring of the occurrence of mycotoxins in beers from market retail

This master thesis deals with monitoring of a content of deoxynivalenol, its metabolite deoxynivalenol-3-b-D-glucopyranoside and ochratoxin A in beer samples collected from retail market in the Czech Republic, Poland and Slovakia. The theoretical part describes general characteristics of mycotoxins, its transfer from field barely through malt to beer and its occurrence in beers. Malting process and brewing technology were also mentioned. Subsequently possibilities for a determination of the mycotoxins by the chromatografic and immunochemical method were presented. The experimental section describes analysis of 30 samples of beer. The analyses were conducted using ultra high-performance liquid chromatography with fluorimetric detection (UPLC/FLR) for ochratoxin A and high-performance liquid chromatography coupled with mass spectrometer (HPLC/MS) for deoxynivalenol and its metabolite. Ochratoxin A was detected in 25 of the 30 samples in concentration range of 0,6 - 82,5 ng·l-1. Deoxynivalenol was found in 24 of the 30 samples with concentration range of 2,29 - 12,57 ug·l-1 and deoxynivalenol-3-b-D-glucopyranoside was occure in 19 of the 30 samples in concentration range of 2,45 - 12,47 ug·l-1. It was also assessed the relationship between beer gushing and presence of mycotoxins in beer. No connection between the parameters has been found. Consequently it is not possible to predict beer gushing from the presence of mycotoxins