Stanovení residuí fluorochinolonů v půdách metodou LC-FD

7. Abstract Several pharmaceutics used in human and veterinary medicine could be detected in environment. To these compounds belongs Fluoroqinolones. Our work observed 4 fluoroqinolons (ofloxacin, norfloxacin, ciprofloxacin, enrofloxacin) in soils sample. Fluoroquinolones were determinate by LC-FD. For extraction was used mixture of organic compounds (methanol: acetone; 1:1) and 1.0g of EDTA. Sample was 15 minutes shacked, 15 minutes in ultrasonic bath and 15 minutes of centrifugation. After that organic solution was evaporated by gentle stream of nitrogen in water bath. Rest from evaporation was dissolved in mQ-H2O and clean-up was done. After evaporation after clean-up the rest was dissolved in mobil phase. Mobil phase was H3PO4: MeOH: ACN (920: 70: 10) and flow was 1.4mL/min. Limit of quantification (LOQ) is For OFLO 5mg/L, for NOR is 0.083mg/L, for CIP is 0.116mg/L and for ENRO is 0.125mg/L. Mean recoveries ranged between 75% to 121%, for OFLO, NOR, CIPRO and ENRO. At the beginning we tried also acidic type of extraction. Extraction was made with HCL, H2SO4, H3PO4 and HNO3. In all cases we add EDTA because of interferences with other substances in samples. We find out, that no complex is build between flouroquinolones and EDTA. For clean- up from soil samples is better OASIS column HLB 6cc/200mg...