Analytical evaluation of biologically active compounds using liquid chromatography.

Disertační práce Summary 133 This dissertation thesis is focused on the analytical evaluation of biologically active compounds with the aid of High Performance Liquid Chromatography, especially with selective and sensitive detection performed by mass and coulometric detector. In the theoretical part of this dissertation thesis, there is attention paid to the importance and status of mass spectrometry as a tool of the modern analytical method of the detection. In this dissertation thesis, there is also a view of electrochemical methods and applications, focusing on coulometric measurements in flow systems. The results of the experimental work have been compiled and presented in three articles published in scientific impact journals. The list of reached results is following: The first article deals with a comparison of four chromatographic columns for their use in bioanalytical studies. Numerous HPLC columns with different bonded stationary phases are nowadays available, and producers continue to expand the range of columns they offer. Four real mixtures of biologically active compounds were used for the testing. Every mixture contained a parent active compound, its real metabolites and proper internal standard. Measurements were performed on two monolithic and two particle-packed columns with quite new..