The effect of dicrocoeliosis on biotransformation of anthelmintics in mouflon

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Field: Patobiochemistry and Xenobiochemistry Candidate: Mgr. Veronika Srpová Consultant: Ing. Barbora Szotáková, Ph.D. Title: The effect of dicrocoeliosis on biotransformation of anthelmintics in mouflon Parasitic infections can modify the host's ability to metabolize drugs and other xenobiotics by altering the biotransformation enzymes. These changes may have various pharmacological, toxicological or physiological consequences. In our study, in vitro metabolism of albendazole, albendazole sulfoxide and flubendazole was tested and compared in non-infected mouflons and in mouflons infected by lancet fluke (Dicrocoelium dendriticum). Two groups of 5-7 years old mouflon ewes (Ovis musimon) were used for our study. Animals in the first group were parasitologically negative and the second group consisted of fluke positive animals. Subcellular fractions of liver homogenates from non-infected and Dicrocoelium-infected mouflons were isolated. The hepatic microsomal and cytosolic fractions were incubated with albendazole, albendazole sulfoxide or flubendazole. The anthelmintics and their metabolites were analyzed using HPLC. Apparent kinetic parameters (Vmax and Km) of biotransformation reactions were..