Use of NMR in structural analysis of natural products

This Diploma work is dedicated to the determination of the structure of unknown substances. A large number of alkaloids was isolated from an Eschscholtzia Californica plant (Papaveraceae) at the Department of Pharmaceutical Botanic and Ecology of the Pharmaceutical Faculty in Hradec Králové. Some of them were passed to the Laboratory of the structure and interaction of the biological aktive molecules along with the request for the structure analysis.The main object of this Diploma work was the identification of three of unknown subjects by the help of NMR analysis. The structures of unknown substances were identified on the basis of the NMR experiments (1 H-NMR, 13 C-NMR, DEPT, ghmqc, ghsqc, ghmqfcops a dpfgnoe). These structures were confirmed based on the comparison with the data provided in the subject publications. Now identified substances belong to isochinoline alkaloids. Namely, it is scoulerine and retikuline. The third substance was not specified in the subject publication