Application of modern technologies for treatment of biological samples for HPLC analysis.

This diploma thesis is dealing with a using of new technologies for the treatment of biological material in the HPLC analysis. The main aim of this thesis was miniaturization of solid phase extraction process of blood serum on classical SPE columns to SPE plates for determination of liposoluble vitamins A and E by high performance liquid chromatography. The new extraction method for vitamin A and E in blood serum using the modern format of SPE plate was developed and optimized. This new method mineralizes a blood serum volume, makes sample treatment easy and can speed up the analysis