Determination of nitrate and nitrite by SIA method.

New SIA method was developed for simultaneous determination of nitrites and nitrates. Nitrites were analyzed by Griess reaction. Nitrates were determined after their reduction to nitrites in cadmium column. Absorbance was measured at maximum of absorption 540 nm. Optimal conditions for analysis: speed of peristaltic pump is 40 % of its maximal speed, speed of syringe pump is 50 µl.s-1 , sample volume of nitrites and nitrates is 150 µl. Optimal time for reduction of nitrates is 0 sec, it means during their flow through cadmium column. Method was used for analysis of nitrites and nitrates in samples of waters: Fromin, Bonaqua, Rajec, Mattoni and tap water in Hradec Králové. Obtained values were compared with values declared by producers. New SIA method for simultaneous determination of nitrites and nitrates is used in subject Special methods of instrumental analysis as a new practical task