Determination of clotrimazol and benzylalcohol in vaginal cream by means of HPLC

HPLC determination of clotrimazol and benzyl alcohol in vaginal cream Academic dissertation Klára Chmurová Charles University in Prague, Pharmaceutical Faculty in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control The task was to find out a method of simultaneous determination of clotrimazol and benzylalcohol in a vaginal cream. We were looking for proper modification of the pharmaceutical preparation (vaginal cream), then optimization of chromatographic conditions and validation of this HPLC method, too. The best results were obtained with DISCOVERY® HS F5, 5 µm, 4,5 × 150 mm together with the pre-column DISCOVERY® HS C18, 5 µm, 2,1 × 20 mm, mixture of acetonitril and buffer (K2HPO4 0,05mol/l, pH = 6,1 set up by 85% H3PO4) 60 : 40 (v/v) was used as the mobile phase, flow rate was 1 ml/min. Detection was realized by UV detector with wave length 223 nm. Time of analysis was shorter than 10 min