Chemiluminescence determination of propylgallate using the flow injection analysis

7 Summary The literature was searched for articles dealling with various ways of determining propylgallate and articles concerning the possible use of the FIA method with chemiluminescent detection in pharmaceutical analysis. Measurement conditions were optimized. Appropriate device parameters, time and flow rate were set. The optimum composition of the carrier stream was found and the influence of some substances to enhance chemiluminescence was tested. The effect on increasing the intensity of CL was detected for sodium polyphosphate, which increased 2.3-fold CL intensity. Under optimum conditions the calibration curve was measured without addition of PFS and the results were evaluated. Measurement conditions have been modified and calibration curve with the addition of PFS was evaluated. Using the calibration curve with the addition of PFS the substance of propylgallate (Aldrich Company) and pharmaceutical formulation were analyzed. Comparative method was chosen - Chelatometric titration. Also, using this method the substance propylgallate (Aldrich company) and formulation were assayed. The results obtained by FIA with chemiluminescent detection and chelatometric titration were statistically evaluated using Student's test and Moore's t test and thus their comparability was demostrated