In vitro evaluation of hydroalcoholic leaf extract of chenopodium album on growth of trichomonase vaginalis

Background and purpose: Trichomonas vaginalis, is one the most common sexually transmitted diseases (STD) in women. Although metronidazole is the drug of choice for trichomoniasis, but due to its side effects attempts have been made to explore an alternative drug particularly with herbal source. Therefore, this study aimed to determine in vitro activity of hydroalcoholic extracts of Chenopodium album (C. album) leaf on the growth of Trichomonas vaginalis. Materials and methods: The plant (C.album) was approved in herbarium and hydro-alcoholic extracts were prepared. The experiment was done using 24 wells cell culture plate. In each well, 200 µl of modified axenic TYM culture medium and 200 µl of different concentrations (5, 37, 75, 150, 300, 600, 1200 µg/mL) of the plant extract was added. They were incubated for 24 and 48 hours and then the plates were incubated at 37 °C. The experiment was performed as a double blind design and in triplicate. Then growth inhibition percent (GI%) of the parasites was evaluated in both times and in different concentrations of plant leaf extract. Results: Compared with metronidazole, the 600 and 1200 µg/mL concentrations of hydro alcoholic leaf extracts of C. album showed 96% and 100% inhibitory effects on the growth of trophozoeites of T. vaginalis in 48 hours, respectively. Conclusion: C. album as an herbal native plant with ant-trichomonas activity is favorable and could be a candidate for in vivo researches on trichomoniasis in future. © 2017, Mazandaran University of Medical Sciences. All rights reserved