Gas Chromatography-Mass Spectrometry Analysis of Viburnum Opulus (L) Extract and its Toxicity Studies in Rats

Objectives: This study was aimed at establishing the antimicrobial and phytochemical profiles of Viburnum opulus (L) as well as the safety potential of the extract in albino Wistar rats. Methods: Ethanol, n-hexane, ethyl acetate, butanol, and water fractions were prepared for both phytochemical assessments using gas chromatography- mass spectrometry analysis (GC-MS). Five groups of seven rats were used for the study. Group A received distilled water (control), while Groups B to E were treated, respectively, with 250, 500, 1000, and 1500 mg/kg body weight of V. opulus extract by abdominal canalization for 28 days. Blood samples were obtained for biochemical analyses, and the liver tissues were further processed for histological studies. Results: The GC-MS spectra revealed the existence of various phytoconstituents such as neophytadiene, germaciene, and caryophyllene among others. High-density lipoprotein and albumin were significantly (p