Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis
- Taylor, Edward J.
- Charnock, Simon J.
- Colby, John
- Davies, Gideon J.
- Black, Gary W.
Publication date
August 2001
DOI Publisher
International Union of Crystallography (IUCr) ISSN
0907-4449 Language
English Citation count (estimate)
4Abstract
The enzyme 6-phospho-3-hexulose isomerase (YckF) from Bacillus subtilis has been prepared and crystallized in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop method at 291 K using polyethylene glycol 2000 monomethylether as precipitant. They diffract beyond 1.7 A using an in-house Cu Kalpha source and belong to either space group P6(5)22 or P6(1)22, with unit-cell parameters a = b = 72.4, c = 241.2 A, and have two molecules of YckF in the asymmetric unit
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