Pengaruh Pemberian Ekstrak Etanol, Fraksi Air, Dan Fraksi Etil Asetat Bunga Rosela (Hibiscus Sabdariffa L.) Terhadap Kelarutan Batu Ginjal Kalsium Secara in Vitro

Kidney stone is one of diseases that found more in Indonesia. Kidney stone is formed by foods contained many calcium compound. Kidney stone is one of kidney disfunction that often happens. Rosela flower (Hibiscus Sabdariffa L.) is one of plants that can be used to drop kidney stone. This research has aimed to know ethanol extract, water fraction and ethyl acetate fraction capability in dissolving kidney stone in vitro. Rosela flower was extracted by ethanol 70% in soxhlet. Ethanol extract obtained was fractionated by ethyl acetate and water, so it was obtained ethanol extract, water fraction, and ethyl acetate fraction. Identification of flavonoid content in rosela flower was done by thin layer chromatografi. Qualitative analysis of kidney stone was done by infra red spectrophotometer to know that there was calcium in kidney stone. Calcium kidney stone was submerged in extract ethanol, water fraction and ethyl acetate fraction for 6 hours, at temperature 37°C and at concentration 2, 4, 6, 8, and 10% v/v. Capability in dissolving at ethanol extract, water faction, and ethyl acetate fraction were measured by AAS method and was analyzed by anava one way with confidence level 95%. The result of research showed that 10 % of ethanol extract is the highest concentration to dissolve Ca in kidney stone followed by water fraction 84,15 ppm and ethyl acetat fraction 30,64 ppm. It was concluded that ethanol extract, water fraction and ethyl acetate fraction of rosela flower were capable to dissolve kidney stone. Th