A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods and emphasizes the potential of HRM for genotyping short sequence repeats in other species. Copyright © 2007, American Society for Microbiology. All Rights Reserved
High resolution melting (HRM) analysis is gaining prominence as a method for discriminating DNA sequ...
High-resolutionmelting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence...
Mutations in simple sequence repeat tracts are a major mechanism of phase variation in several bacte...
A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR)...
A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR)...
The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jej...
The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jej...
Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countrie...
Multi-locus sequence typing (MLST) has emerged as the state-of-the-art method for resolving bacteria...
Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countrie...
. lineages might be originated from certain areas along those routes.CRISPR can provide important in...
Abstract Background Campylobacter jejuni is the leading bacterial pathogen that causes foodborne ill...
Campylobacter spp. are important causes of bacterial gastroenteritis in humans in devel-oped countri...
Campylobacter infection is a common cause of bacterial gastroenteritis in humans and remains a signi...
Mutations in simple sequence repeat tracts are a major mechanism of phase variation in several bacte...
High resolution melting (HRM) analysis is gaining prominence as a method for discriminating DNA sequ...
High-resolutionmelting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence...
Mutations in simple sequence repeat tracts are a major mechanism of phase variation in several bacte...
A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR)...
A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR)...
The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jej...
The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jej...
Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countrie...
Multi-locus sequence typing (MLST) has emerged as the state-of-the-art method for resolving bacteria...
Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countrie...
. lineages might be originated from certain areas along those routes.CRISPR can provide important in...
Abstract Background Campylobacter jejuni is the leading bacterial pathogen that causes foodborne ill...
Campylobacter spp. are important causes of bacterial gastroenteritis in humans in devel-oped countri...
Campylobacter infection is a common cause of bacterial gastroenteritis in humans and remains a signi...
Mutations in simple sequence repeat tracts are a major mechanism of phase variation in several bacte...
High resolution melting (HRM) analysis is gaining prominence as a method for discriminating DNA sequ...
High-resolutionmelting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence...
Mutations in simple sequence repeat tracts are a major mechanism of phase variation in several bacte...