Rosa canina extracts have antiproliferative and antioxidant effects on Caco-2 human colon cancer

The in vitro antiproliferative and antioxidant effects of different fractions of Rosa canina hips on human colon cancer cell lines (Caco-2) was studied. The compounds tested were total extract (fraction 1), vitamin C (fraction 2), neutral polyphenols (fraction 3) and acidic polyphenols (fraction 4). All the extracts showed high cytotoxicity after 72 h, both low and high concentrations. The flow cytometric analysis revealed that all the fractions produce disturbances in the cell cycle resulting in a concomitant cell death by an apoptotic pathway. Changes in the redox status of Caco-2 cells in response to Rosa canina hips were determined. Cells were exposed to hydrogen peroxide in presence of plant fractions and the production of Reactive Oxygen Species (ROS) was significantly decreased. Therefore, our data demonstrate that rosehip extracts are a powerful antioxidant that produces an antiproliferative effect in Caco-2 cells. Therefore, these results predict a promising future for Rosa canina as a therapeutic agent. Thus, this natural plant could be an effective component of functional foods addressed towards colorectal carcinoma.This study was supported by the projects: the Ministry of Science and Innovation (SAF2013-41651), founds from Aragón Regional Government (A-32) and CIBER (Centros de Investigación Biomédica en Red) Sub-program (CIBERobn, CB06/03/1012,) by the Instituto de Salud Carlos III and Proyecto ELENA, EFA 220/11 ELENA.Peer Reviewe