Development of a HILIC-MS method for the analysis of goat colostrum oligosaccharides

Resumen del trabajo presentado a las 14as Jornadas de Análisis Instrumental celebradas en Barcelona (España) del 1 al 3 de octubre de 2014.Some studies reveal remarkable similarities between human milk and goat milk oligosaccharides (GMO). GMO are composed by fucosylated and sialylated oligosaccharides whose beneficial properties, such as anti-inflammatory effect or modulation of the immune system, are well known. Nevertheless, studies about characterization of GMO are scarce and mainly focused on a qualitative analysis of the main oligosaccharides. Moreover, to the best of our knowledge, no relationship between oligosaccharides composition of goat milk and the different stages of lactation has been reported. In this work, a recent developed method based on hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) has been applied to the characterization of GMO. Relationships between oligosaccharide composition and the different stages of lactation (1 and 2 days colostrum, 10, 20, 30 and 40 days) have been established. GMO were extracted from milk samples by precipitation of fat and proteins using the methodology described by Martínez-Ferez et al. GMO analysis was performed on an Agilent 1200 series HPLC system coupled to an ESI quadrupole HP-1100 mass detector (Hewlett-Packard, Palo alto, CA, USA) using an ethylene bridge hybrid with trifunctionally-bonded amide phase column (BEH X-Bridge, Waters) and a binary mixture of acetonitrile:water with 0.1% ammonium hydroxide as mobile phase. Acid and neutral oligosaccharides were detected in goat milk samples. In general, GMO concentrations decreased during the lactation period studied; the highest concentrations were found in 1 and 2 days colostrum samples. Differences in chromatographic profiles and relative abundances of the detected GMO were observed.This work was funded by Junta de Andalucía (project POII10-0178-4685) and CSIC (project i-link0827).Peer Reviewe