MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments Stephen A Bustin1 , Jean-François Beaulieu2 , Jim Huggett3 , Rolf Jaggi4 , Frederick SB Kibenge5 , Pål A Olsvik6 , Louis C Penning7 and Stefan Toegel8 1 Centre for Digestive Diseases, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, 3rd Floor Alexandra Wing Royal London Hospital, London, UK 2 Canadian Institutes for Health Research Team on the Digestive Epithelium, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada 3 Molecular and Cell Biology, LGC, Queens Rd, Teddington, Middlesex TW11 OLY, UK 4 Department of Clinical Research, University of Bern, Murtenstrasse 35, CH-3010 Bern, Switzerland 5 OIE Reference Laboratory for ISA, Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, P.E.I., C1A 4P3, Canada 6 National Institute of Nutrition and Seafood Research, 5817 Bergen, Norway 7 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, the Netherlands 8 Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria author email corresponding author email BMC Molecular Biology 2010, 11:74doi:10.1186/1471-2199-11-74 The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2199/11/74 Received: 13 September 2010 Accepted: 21 September 2010 Published: 21 September 2010 © 2010 Bustin et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited