Changes in P-31-relaxation times during organ preservation: Observations on cold stored human donor livers

During cold preservation for transplantation the tissue hydration state changes, It is not known whether such changes lead to altered relaxation times of P-31 nuclei with potential consequences for the quantification of tissue metabolites, Therefore, P-31 spectroscopic and proton T-1 relaxometric measurements were performed on 42 isolated human donor livers shortly before implantation, The results demonstrate that P-31 T-1 relaxation times change during preservation for clinical transplantation, thus quantification of tissue metabolites in cold stored donor livers may be in part dependent on the tissue hydration state, Furthermore, it appeared that changes in tissue hydration state especially affect the physico-chemical characteristics of the intracellular fluid compartment. This study indicates that reliable spectroscopic quantification of tissue metabolites, particularly during sequential spectroscopic measurements in cold stored donor organs is best warranted under fully relaxed conditions. (C) 1997 Elsevier Science Inc