Comparative urine analysis by liquid chromatography-mass spectrometry and multivariate statistics: Method development, evaluation, and application to proteinuria

Kemperman, Ramses F. J.
Horvatovich, Peter L.
Hoekman, Berend
Reijmers, Theo H.
Muskiet, Frits A. J.
Bischoff, Rainer

Publication date

January 2007

DOI

Abstract

We describe a platform for the comparative profiling of urine using reversed-phase liquid chromatography-mass spectrometry (LC-MS) and multivariate statistical data analysis. Urinary compounds were separated by gradient elution and subsequently detected by electrospray Ion-Trap MS. The lower limit of detection (5.7-21 nmol/L), within-day (2.9-19%) and between-day (4.8-19%) analytical variation of peak areas, linearity (R-2: 0.918-0.999), and standard deviation for retention time (<0.52 min) of the method were assessed by means of addition of seven 3-8 amino acid peptides (0-500 nmol/L). Relating the amount of injected urine to the area under the curve (AUC) of the chromatographic trace at 214 nm better reduced the coefficient of variation (...