NF-kappa B signaling pathway is involved in growth inhibition, G2/M arrest and apoptosis induced by Trichostatin A in human tongue carcinoma cells

The HDAC inhibitor Trichostatin A (TSA) exhibits antiturnour activity in various tumour cells. However, little is known about the effect of TSA on growth of human tongue carcinoma cells. In this study, we observed that TSA concentration-dependently inhibited growth of human tongue carcinoma Tca8113 cells by inducing G2/M arrest, apoptosis, up-regulation of the pro-apoptotic protein Bax and down-regulation of the anti-apoptotic proteins Bcl-2 and Bcl-XL which are regulated by the transcription factor nuclear factor (NF)-kappa B. Coincident with this observation, TSA induced a concentration-dependent reduction of constitutive and tumour necrosis factor (TNF)-alpha-induced NF-kappa B activation in Tca8113 cells. This induction was correlated with decreased phosphorylation and increased expression of inhibitors of NF-kappa B (I kappa B)alpha induced by TSA. Overall, our results indicate inhibition of NF-kappa B activation contributes, at least partially, to the antiturnour activity of TSA in human tongue carcinoma cells. (c) 2006 Elsevier Ltd. All rights reserved