A number of phosphoinositide-specific phospholipases C (PLC) of different species have recently been cloned. The predicted amino acid sequences of these isoforms contain two highly conserved domains. Here we report the identification of a PLC gene of Dictyostelium by using the polymerase chain reaction. Primers were designed coding for highly conserved amino acid regions located within one of the conserved domains of PLCs. Cloning and sequencing of the polymerase chain reaction product revealed one unique PLC-like sequence. This sequence was used to screen a library and isolate several overlapping cDNA clones. The complete cDNA was expressed in Dictyostelium cells resulting in increased basal levels of inositol 1,4,5-trisphosphate and enhan...
Phosphoinositide-specific phospholipase C (PLC) is dependent on Ca2+ ions for substrate hydrolysis. ...
Phosphoinositide-specific phospholipases C (PLCs) play an important role in many cellular responses ...
The cellular slime mould Dictyostelium discoideum shows several responses after stimulation with the...
A number of phosphoinositide-specific phospholipases C (PLC) of different species have recently been...
A number of phosphoinositide-specific phospholipases C (PLC) of different species have recently been...
A number of phosphoinositide-specific phospholipases C (PLC) of different species have recently been...
A number of phosphoinositide-specific phospholipases C (PLC) of different species have recently been...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
Phosphoinositide-specific phospholipase C (PLC) is generally considered to be one of the central eff...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
Phosphoinositide-specific phospholipase C (PLC) is dependent on Ca2+ ions for substrate hydrolysis. ...
Phosphoinositide-specific phospholipases C (PLCs) play an important role in many cellular responses ...
The cellular slime mould Dictyostelium discoideum shows several responses after stimulation with the...
A number of phosphoinositide-specific phospholipases C (PLC) of different species have recently been...
A number of phosphoinositide-specific phospholipases C (PLC) of different species have recently been...
A number of phosphoinositide-specific phospholipases C (PLC) of different species have recently been...
A number of phosphoinositide-specific phospholipases C (PLC) of different species have recently been...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
Phosphoinositide-specific phospholipase C (PLC) is generally considered to be one of the central eff...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
The micro-organism Dictyostelium uses extracellular cAMP to induce chemotaxis and cell differentiati...
Phosphoinositide-specific phospholipase C (PLC) is dependent on Ca2+ ions for substrate hydrolysis. ...
Phosphoinositide-specific phospholipases C (PLCs) play an important role in many cellular responses ...
The cellular slime mould Dictyostelium discoideum shows several responses after stimulation with the...