IGH switch breakpoints in Burkitt lymphoma: Exclusive involvement of noncanonical class switch recombination

Most chromosomal t(8;14) translocations in sporadic Burkitt lymphomas (BL) are mediated by immunoglobulin class switch, recombination (CSR), yet all tumors express IgM, suggesting an incomplete or exclusively monoallelic CSR event. We studied the exact configuration of both the nontranslocated IGH allele and the MYG/IGH breakpoint by applying a combination of low- and high-resolution methods (interphase FISH, DNA fiber FISH, long-distance PCR, and Southern blotting) on 16 BL. IGH class switch events involving the nontranslocated IGH allele were not observed. Thirteen cases had MYG/IGH breakpoints in or nearby IGH switch (S) sites, including five at S mu, three at S gamma and five at S alpha. All eight translocations with a breakpoint at S gamma or S alpha were perfectly reciprocal, without deletion of C mu-C delta or other CH elements. Internal S mu deletions claimed to be a marker for CSR activity and implicated in stabilization of IgM expression were found in BL but did riot correlate with downstream translocation events. This study shows that switch breakpoints in sporadic BL are exclusively resolved by a noncanonical recombination mechanism involving only one switch region. (c) 2006 Wiley-Liss, Inc