Temperature-driven self-actuated microchamber sealing system for highly integrated microfluidic devices

We present here a novel microchamber sealing valve that is self-actuated by a pressure change during the temperature change in the thermal activation of reactions. Actuation of our valve requires only the use of the same heating device as employed for the reactions. A thermoplastic UV-curable polymer is used as a device material; the polymer allows realization of the temperature-driven valve actuation as well as the fabrication of multi-layered devices. The self-actuated valve achieves effective sealing of the microchamber for the polymerase chain reaction (PCR) even at 90 degrees C, which is essential for developing highly parallel PCR array devices without the need for complicated peripherals to control the valve operatio