Investigation on carob seed germination under controlled conditions

In this study, the effects of some pre-treatments on seed germination of wild carob seeds were investigated under two different conditions (germination cabinet and greenhouse). Twenty two pre-treatments were applied to the seeds. Pre-treated and control seeds were placed under dark conditions at 25°C. All treated seeds were germinated at 27°C temperature in the germination cabinet and greenhouse conditions. Seed germination rate was determined according to the pre-treatments for both conditions. The results showed that if the seeds were soaked in pure (98 %) or diluted sulphuric acid (H2SO4, 40 %, 90 %) for 30 minutes then kept in water for 2 days or just soaking the seeds in H2SO4 sulfuric acid for 30 minute gave the best results in terms of seed germination as the seed germination rate was over 90 %. The lowest germination rate under both conditions was observed in the control and soaking the seeds in 60 % H2SO4 for 30 minutes. On the other hands, seeds soaked in 60 % H2SO4 and then kept in water for 2 days did not germinate as they lost germination ability. The highest germination rates were recorded after ten days of pre-treatments in both conditions