PHAEOACREMONIUM SPECIES IN GRAPEVINES WITH ESCA AND PETRI DISEASE SYMPTOMS

Gljive iz roda Phaeoacremonium ubrajaju se među uzročnike eske i eski sličnih bolesti vinove loze. Od 2009. do 2014., u šest vinograda (Kutjevo, Ilok, Nadin, Buje i Vukomeričke Gorice) sakupljeno je 16 uzoraka trsova sa simptomima Petrijeve bolesti (mladi vinograd u Nadinu) ili eske (ostali vinogradi). U 13 uzoraka nađene su vrste iz roda Phaeoacremonium, od toga u pet uzoraka u savršenom stadiju (periteciji), a u ostalih osam uzoraka u kondijskom stadiju. Analizom izolata na temelju morfologije i analizom sekvenci ITS1-5.8S-ITS2 ribosomske DNA utvrđeno je da izolati pripadaju vrstama Phaeoacremonium mortoniae (11 izolata), P. aleophilum (jedan izolat) i P. rubrigenum (jedan izolat). Za pet izolata koji su dobiveni iz peritecija nađenih na uzorcima vinove loze utvrđeno je da pripadaju vrstama Togninia fraxinopennsylvanica (P. mortoniae), T. minima (P.aleophilum) i T. Rubrigena (P. rubrigenum). Rezultati predstavljaju prvi nalaz savršenih stadija Phaeoacremonium vrsta na vinovoj lozi u Hrvatskoj i upućuju na mogućnost da se spomenuti patogeni, uzročnici eske i eski sličnih bolesti, mogu razmnožavati konidijama i askosporamaFungi from the genus Phaeoacremonium are known to be among the causal agents of grapevine esca and esca-like diseases. From 2009 to 2014, 16 symptomatic samples of grapevine with esca or Petri disease symptoms were collected in Nadin (Petri disease symptoms), Kutjevo, Ilok, Buje and Vukomeričke Gorice (esca symptoms). Phaeoacremonium species were found in 13 samples. Perithecial stage (Togninia) was found in five samples, while only conidial stage was recorded in the other eight samples. Morphological analysis and phylogenetic analysis of ITS1-5.8S-ITS2 ribosomal DNA sequences showed that 11 isolates can be identified as Phaeoacremonium mortoniae, one isolate as P. aleophilum and one isolate as P. rubrigenum. Five isolates derived from perithecia found on grapevine wood were confirmed as Togninia fraxinopennsylvanica (P. mortoniae), T. minima (P. aleophilum) and T. rubrigena (P. rubrigenum). This is the first record of Phaeoacremonium teleomorphs on grapevine in Croatia, which indicates that these causal agents of esca and esca-like diseases can reproduce both with conidia and ascospores