Effect of Taxusbaccata leaves fractions on Trichomonasvaginalis growth in culture medium

Abstract Background & aim: Tichomoniasis is a common sexually transmitted disease (STD) caused by a protozoan parasite called Trichomonas vaginalis. Metronidazole, with vast side effects, is the main treatment for this infection. The aim of this study was to investigate the effect of the extract fractions of Taxusbaccata, on Trichomonas vaginalis in cultured medium. Methods: In the present study, asetonia dichloromethane extract of Taxusbaccata and hydroalcoholic fractions of 60% and 90% were collected and then dried. Their anti-trichomonas effect at doses of 200, 300, 400 and 500 μg/ml at 24, 48 and 72 hours on the TYIS33 culture media were studied. Data were analyzed using ANOVA test. .Results: The crude extract and fractions of 60% at different times at concentrations of 200, 300, 400 and 500 μg/ml showed higher anti-parasitic effects than 90% (p < 0.05). At concentration of 200 μg/ml of extract of 60% which causing 100% inhibition growth but the fraction of 90% showed 60% of inhibition growth. Conclusion: The effect of Taxusbaccata on Trichomonas may be a good alternative candidate for metronidazole. Key words: Taxusbaccata, Trichomonas vaginalis, Fracction