Effect of hypoxia-inducible factor-1α induction by CoCl₂ on breast cancer cells survival: influence of cytochrome-c and survivin

<p><strong>Background:</strong> Tumor tissue usually became hypoxic due to disruption of oxygen supply. Adaptation response to hypoxia is mediated by transcription factor, hypoxia- inducible factor-1α (HIF-1α). HIF-1α signaling is known to increase the expression of pro-apoptotic protein cytochrome-c, and anti- apoptotic survivin. In this study we wanted to analyze the role of HIF-1α on breast cancer cells survival through pro-apoptosis cytohrome-c and anti-apoptosis survivin regulation.</p><p><strong style="font-size: 10px;">Methods:</strong><span style="font-size: 10px;"> Breast cancer cell lines T47D were induced by CoCl</span>₂<span style="font-size: 10px;"> then harvested to analyze the expression of HIF-1α, protein cytochrome-c, mRNA survivin and cell viabilities.</span></p><p><strong>Results:</strong> HIF-1α induction by CoCl₂ causes the increase of protein and mRNA of HIF-1α, cytochrome-c protein, and survivin mRNA, but does not cause the changes in cell viability.</p><p><strong>Conclusion:</strong> HIF-1α induction have no effects on breast cancer cell line T47D viabilities due to the balance regulation between pro-apoptosis expression cytochrome-c and anti-apoptosis survivin.