Tingkat Maturasi in vitro Oosit Kambing dalam Medium dengan Suplementasi Serum dan Albumin

<div>The present study aimed to study the effect of ?maturation media? on maturation rate of goat oocytes</div><div>after in vitro maturation. Goat ovaries were collected from a slaughter house in Godean, Sleman. Immediately</div><div>after slaughter the ovaries were collected, rinsed with physiological NaCl three times then placed in a</div><div>flask containing the NaCl solutions and hept at 36-370C before transportaion to the laboratory. Oocytes</div><div>were observed under stereo microscope and its quality was classified into A, B, and C. Oocytes in vitro</div><div>maturaion (IVM) was performed in TCM-199 media suplemented with : a) 0.4 mg/ml bovine serum albumin</div><div>(BSA); and b) 10% newborn calf serum (NCS) then incubated at 38.50C with 5% CO2 for 24-27 h. Following</div><div>this, oocytes were observed under inverted microscope for first polar body extrusion, then stained with</div><div>aceto-orcein in order to evaluate nuck or maturation. The nuclear maturation stages including : germinal</div><div>vesicles (GV), germinal vesicles break down (GVBD), metaphase I, apaphase I, telophase I and metaphase</div><div>II, respectivelly. The overall results showed that 74-74%, 52-66.6% and 21.5-23.8% of oocytes quality A, B,</div><div>and C reached maturation at metaphase II, respectivelly. There were no significant differences in oocytes</div><div>maturation using media supplemented with either BSA or NCS.