Analisis Sekuen Probe Gena Shiga Like Toxin-2 dari Isolat Lokal Escherichia coli O157:H7 (PROBE SEQUANCE ANALYSIS OF SHIGA LIKE TOXIN-2 GEN FROM ESCHERICHIA COLI O157:H7 LOCAL ISOLATES)

A Shiga-like toxin producing Escherichia coli O157:H7 was detected in faecal samples of cattle, andhuman as well as in beef. The performance of agent indicated that it has been identified as harmful andoften life-threatening zoonotic agent. It is therefore important to analysed the genetic characteristic ofShiga toxin Escherichia coli (STEC) and to develop a diagnostic probe in order to optimalized of diagnostictest for the agent. The study was started by amplifiying stx2 gene, purifying of PCR product, sequencingof stx2 gene, analyzing of phylogenetic tree, and finally by analyzing of diagnostic probe candidate.Homology study showed that the genetic sequence of the local isolate of E. coli O157:H7 i.e SM25(1)isolated from cattle feces has a genetic and fuctional similarity with the control isolate i.e E. coli O157:H7ATCC 43894 originated from human. Further study showed that a probe with foreward primer sequanceof 5’-AATTTATATGTGGCCGGGTTC-‘3 which were respectively designed as a PFS and PRS 176 bp product.Appeared to be potential candidate of diagnostic probe for the agent