Expression of bone morphogenetic protein-2 after using chitosan gel with different molecular weight on wound healing process of dental extraction

<span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Background:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> Bone morphogenetic protein-2 (BMP-2) is bone stimulator which capable of inducing differentiation of mesenchymal </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>cells into osteoblast, stimulating bone formation in wound healing process of dental extraction. Chitosan is polymer composed N-acetylD-glucosamine unit that has been used in various applications in wound healing process and bone tissue engineering. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Purpose: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>objective of this research was to analyzed expressions of BMP-2 for 7,14 and 21 days after using chitosan gel with different molecular </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>weight on wound healing process of dental extraction. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Method:</strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em> The research was an experimental laboratory study. Rattus nornegicus </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>strain wistar male, aged 8-16 weeks, divided into 3 treatment groups namely group 1 and II which given chitosan gel 1 % with high </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>and low molecular weight and group III as control which were not given chitosan gel. Chitosan gel were applied into the socket of </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>dental extraction. Rat was decaputated 7,14 and 21 days after chitosan gel application and the jaw in the treated regions and control </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>group were cut for immunohistochemical examination to observe BMP-2. Data were analyzed using ANOVA test. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Result: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The result </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>of this research showed significant differences on BMP-2 for 7,14 and 21 days observation (p&lt;0,05). The increasing of BMP-2 were </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>found in the group which given chitosan gel with high molecular weight. </em><span style="font-family: Tribune-Bold; font-size: 10pt; color: #231f20; font-style: normal; font-variant: normal;"><strong>Conclusion: </strong><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>It may be concluded that chitosan gel with high </em><span style="font-family: Tribune-Italic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>molecular weight can enhance the expresion of BMP-2 on wound healing process ofdental extraction.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span>