In Vitro Cytotoxicity of Plantago australis Ethanol Extract Used as an Anti-Inflammatory for the Treatment of Oral Pathologies

Background Plantago australis Lam. is a plant native to southern Brazil largely used to reduce inflammation and improve the healing process. Interestingly, though, there are no previous studies evaluating the cytotoxicity of its ethanolic extract. Objectives The main objective is to assess in vitro the cytotoxicity of the crude ethanolic extract of P. australis at different concentrations. Materials and Methods The MTT cytotoxicity assay was performed on a 3T3 mouse fibroblast cell line to measure the in vitro cytotoxic activity of the crude formulation. Results In vitro analysis showed that the greater the concentration of the extract, the greater the cytotoxicity. Conclusions The crude extract of P. australis might be cytotoxic at high concentrations as evaluated against the cell line