Healing Effect of PistaciaAtlantica Fruit Oil Extract in Acetic Acid-Induced Colitis in Rats

Background: Considering the anti-oxidant properties of Pistaciaatlanticaand lack of data regarding its efficacy in the treatment of ulcerative colitis, this study aims at investigating the effect of the Pistaciaatlantica fruit extract in treating experimentally induced colitis in a rat model. Methods:Seventy male Sprague-Dawley rats (weighing 220±20 g) were used. All rats fasted 24 hours before the experimental procedure. The rats were randomly divided into 7 groups, each containing 10 induced colitis with 2ml acetic acid (3%). Group 1 (Asacol), group 2 (base gel) and group 7 (without treatment) were assigned as control groups. Group 3 (300 mg/ml) and group 4 (600 mg/ml) received Pistaciaatlantica fruit orally. Group 5 (10% gel) and group 6 (20% gel) received Pistaciaatlantica in the form of gel as enema. Macroscopic, histopathological examination and MDA measurement were carried out. Results:All groups revealed significant macroscopic healing in comparison with group 7 (P<0.001). Regarding microscopic findings in the treatment groups compared with group 7, the latter group differed significantly with groups 1, 2, 4 and 6 (P<0.001). There was a significant statistical difference in MDA scores of the seven treatment groups (F(5,54)=76.61, P