Protein-mediated bridging is ubiquitous and essential for shaping cellular structures in all organisms. Here we dissect this mechanism for a model system: the Histone-like Nucleoid-Structuring protein (H-NS). We present data from two complementary single-molecule assays that probe the H-NS-DNA interaction: a dynamic optical-trap-driven unzipping assay and an equilibrium H-NS-mediated DNA looping scanning force microscopy imaging assay. To quantitatively analyze and compare these assays, we employ what we consider a novel theoretical framework that describes the bridging motif. The interplay between the experiments and our theoretical model not only infers the effective interaction free energy, the bridging conformation and the duplex-duplex...
Chromatin is a higher-order structure that packages eukaryotic DNA. Chromatin undergoes dynamic alte...
<p>(a) When the DNA (yellow chain) is wrapped around the histone octamer (blue), we can represent th...
The bacterial chromosome is organized into loops, which constitute topologically isolated domains. I...
AbstractProtein-mediated bridging is ubiquitous and essential for shaping cellular structures in all...
Both prokaryotic and eukaryotic organisms contain DNA bridging proteins, which can have regulatory o...
AbstractThe histone-like nucleoid structuring protein (H-NS) is a nucleoid-associated protein, which...
The histone-like nucleoid structuring protein (H-NS) is a nucleoid-associated protein, which is invo...
International audienceThis work investigates the interactions of H-NS proteins and bacterial genomic...
The nucleoid-associated protein H-NS is thought to play an essential role in the organization of bac...
Atomic force microscopy (AFM) has proven to be a powerful tool for the study of DNA-protein interact...
Atomic force microscopy (AFM) has proven to be a powerful tool for the study of DNA-protein interact...
Atomic force microscopy (AFM) has proven to be a powerful tool for the study of DNA-protein interact...
Molecular dynamics simulations are used to model proteins that diffuse to DNA, bind, and dissociate;...
AbstractThe histone-like nucleoid structuring protein (H-NS) is a nucleoid-associated protein, which...
Nucleoid-associated proteins are a large family of proteins that are involved in both structuring an...
Chromatin is a higher-order structure that packages eukaryotic DNA. Chromatin undergoes dynamic alte...
<p>(a) When the DNA (yellow chain) is wrapped around the histone octamer (blue), we can represent th...
The bacterial chromosome is organized into loops, which constitute topologically isolated domains. I...
AbstractProtein-mediated bridging is ubiquitous and essential for shaping cellular structures in all...
Both prokaryotic and eukaryotic organisms contain DNA bridging proteins, which can have regulatory o...
AbstractThe histone-like nucleoid structuring protein (H-NS) is a nucleoid-associated protein, which...
The histone-like nucleoid structuring protein (H-NS) is a nucleoid-associated protein, which is invo...
International audienceThis work investigates the interactions of H-NS proteins and bacterial genomic...
The nucleoid-associated protein H-NS is thought to play an essential role in the organization of bac...
Atomic force microscopy (AFM) has proven to be a powerful tool for the study of DNA-protein interact...
Atomic force microscopy (AFM) has proven to be a powerful tool for the study of DNA-protein interact...
Atomic force microscopy (AFM) has proven to be a powerful tool for the study of DNA-protein interact...
Molecular dynamics simulations are used to model proteins that diffuse to DNA, bind, and dissociate;...
AbstractThe histone-like nucleoid structuring protein (H-NS) is a nucleoid-associated protein, which...
Nucleoid-associated proteins are a large family of proteins that are involved in both structuring an...
Chromatin is a higher-order structure that packages eukaryotic DNA. Chromatin undergoes dynamic alte...
<p>(a) When the DNA (yellow chain) is wrapped around the histone octamer (blue), we can represent th...
The bacterial chromosome is organized into loops, which constitute topologically isolated domains. I...