Development and optimization of bioanalytical parameters for the standardization of Trigonella foenum–graecum

Objective: To develop a novel qualitative and quantitative method, which can pave the way for rapid and selective determination of different constituents of Trigonella foenum–graecum (T. foenum–graecum). Methods: Phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and quantitative analysis through HPTLC techniques were performed in the present investigation. Results: Phytochemical analysis showed to contain alkaloid, saponin, tannin, flavonoid, steroid, triterpenoid, glycoside and protein. pH of the 1% solution was found to be 5.65, loss on drying was 4.5%, total ash, solubility in water and in 50% alcohol was 7.11%, 81.00% and 84.25% respectively. Total flavonoid and total phenol content were found to be 1.8% and 0.60% (w/w). Level of different heavy metals and microorganisms were found to be under the limit. Content of quercetin and rutin in the T. foenum–graecum were found to be 0.879% (w/w) and 0.417% (w/w) respectively. Conclusions: In the future, these phytochemical parameters could be used as an important tool for the standardization of T. foenum–graecum