Optimization Making Glucosamine Hydrochloride (HCl GlcN) of Crab Shell Waste through Chemical Hydrolysis

<p>Processing of glucosamine from crab shell chitin can be done by chemical hydrolysis with hydrochloric<br />acid (HCl). The purpose of this research was to determine the effect of chitin immersion by various<br />concentrations of HCL toward glucosamine generated and the selected concentration of HCL to glucosamine<br />chemical hydrolysis process. The material used in this study is crab shells from Betahwalang village, Demak,<br />and the solvent is HCl and NaOH. Parameters are glucosamine yield, melting point, loss on drying (LoD),<br />PH and spectrum analysis with FTIR method. Research using experimental design completely randomized<br />design three times treatment with repetition. The results were analyzed using analysis of variance (ANOVA)<br />to determine the differences between treatments concentration of 27%, 32% and 37%. The selected were<br />tested with honestly significant difference test. The results showed differences in the concentration of HCl<br />used cause differences in the characteristics of the resulting glucosamine. The higher HCl concentrations<br />used, the lower the rendemen results and melting point. The selected HCL treatment concentration was at<br />27% observed from glucosamine yield (18.39%) and the melting point (192-195oC). The treatment of 37%<br />produces LoD (0.647%), pH (4.01) and spectrum glucosamine is an O-H group (3297.75/cm), the group of<br />N-H (1617.53/cm), the group of C-N (1394.94/cm).<br />Keywords: crab shells, chitin, melting point, glucosamine