Effects of Salinity and Drought Stresses on Germination Characteristics of Milk Thistle (Silybum marianum)

Abstract\ud In order to study the germination and seedling growth responses of Milk Thistle as a medicinal plant to salinity and drought stresses, two separate experiments were conducted based on completely randomised design with four replications. In the first experiment, the effect of drought potential levels (0, -1, -2, -3, -4, -5, -6, -7, -10, -15 and -20 bars) due to using polyethilenglycol (PEG) 6000 and the second trial evaluated effects of salinity potential (0, 50, 100, 150, 200, 250 and 300 ml/molar) caused by NaCl were studied on germination characteristics and seedling growth. The results indicated that salinity and drought stresses showed significant effects on germination and seedling growth of milk thistle. The seeds were able to germinate in 300 ml/molar salinity potential and -20 bar drought potential conditions. The effect of both stresses on length and dry weight of seedlings were significant and with increasing salinity and drought stresses, the length and dry weight of radicles and plumules decreased. Increasing drought level, led to higher plumule length reduction compared to radicle length reduction, which shows that milk thistle plumule is more sensitive to droughtness than radicle. Dry weight of seedling at -3 bar drought potential was 50% of control plants.\ud \ud Keywords: PEG, Drought stress, Sodium chloride, Milk thistl