Evaluation of the Potential Use of Agricultural and Forestial Waste in Spawn Production of Medicinal Mushroom (Ganoderma lucidum)

Spawn quality plays an important role in successful production of medicinal mushrooms. In this study, firstly, to determine the optimum temperature for mycelia growth of G. lucidum, four basal media, including seeds of wheat, barley, millet and Abies sp. wood chip were studied separately at 25±1˚C and 29±1˚C. In the second section, in order to achieve suitable the mycelia growth, barley, wheat and millet seeds were mixed with different ratios of agriculture waste including wheat bran (10, 20 and 30% dry weight) and millet peel (20, 40 and 60% dry weight) and also Abies sp wood chips (20, 30, 50 and 60% dry weight) as forestial waste. In the final section, several forestial waste including sawdusts (Platanus orientalis, Acer sp, Robinia peseudoacacia, Ailanthus altissima, Fagus orientals, Alnus subcordata and Populas alba) were used as medium for spawn production. In the first experiment, a higher mycelia growth rate (8.92 mm/day) was obtained by applying wheat seed at 29±1˚C. In the second experiment, the results showed that higher mycelia growth rate was obtained by using wheat with 10% wheat bran (9.66 mm/day). In the final section of spawn production, R. peseudoacacia sawdust (C/N=25.84) was generated higher growth rate (9.36 mm/day). Also, using supplements containing nitrogen (N) such as sawdust and bran, encourage mycelium growth and with increasing C/N ratios more than 61.3 decreased growth rate due to reduce N amount