KAJIAN KEAMANAN ASAP CAIR TEMPURUNG KELAPA UNTUK PRODUK PANGAN

The objective of this research was to study the food satefy of coconut shell liquid smoke for food products by acute toxicity test and identification of volatile compounds by means of Gas Chromatograpy-Mass spectroscopy (GC-MS). Acute toxicity test of these product were assessed by determination of LD50 dose (the single dose which causes the death of half the tset animals) based on OECD 402 (2001) Guidelines for the Testing of Chemicals. Three of mice are used for each step. The dose level to be used is selected of five fixed levels, 0, 50, 500, 5.000, and 15.000mgkg-1 body weight. Result indicated that LD50 dose of this liquid smoke are more than 15.000mg.kg-1 body weight of mice. Based on regulation by the Indonesian Goverenment (Regulation 74/RI/ 2001), liquid smoke with LD50 value more than 15.000mg.kg-1 body weight of mice, this product was no toxic and safe for food products. Identification of volatile compound liquid smoke was start by extracted thes product using dicloromethane as solvent. Result Of GC-MS showed that liquid smoke comprise 40 components. From GC-MS spectra were identified 7 peaks with a higher proportions. They were identified as 2-Metoxypenol (guaiacol), 3,4-Dimethoxyphenol, Phenol, 2-methoxy-4-methyphenol, 4-Ethyl-2-metoxyphenol, 3-Methyphenol, and 5-Methyl-1,2,3-trymethoxy benzene. Neither benzo [a]pyrene is regarded as a marker of the carcinogenic prperties were found in this liquid smoke. benzo [a]pyrene nor other polycyclic aromatic compounds with cacinogenic compounds in food products, althogh in foods the maximum level of 10ug.kg-1 for benzo [a]pyrene has been set by the European Commission. Analytical procedurs based on extarction of the gydrocarbons from the matrix, separation by Gas Chromatography (GC), followed by detection by Mass Spectroscopy (MS), make it possible to determine polycylclic aromatic compounds including benzo[a]pyrene in food product