Determination Total Phosphour of Maize Plant Samples by Continuous Flow Analyzer in Comparison with Vanadium Molybdate Yellow Colorimetric Method

The vanadium molybdate yellow colorimetric method(VMYC method) is regarded as one of conventional methods for determining total phosphorus(P) in plants, but it is time consuming procedure. Continuous flow analyzer(CFA) is a fluid stream segmentation technique with air segments. It is used to measure P concentration based on the molybdate-antimony-ascorbic acid method of Murphy and Riley. Sixty nine of maize plant samples were selected and digested with H2SO4-H2O2. P concentrations in the digests were determined by CFA and VMYC method, respectively. The t test found that there was no any significant difference of the plant P contents measured by the CFA and the VMYC method. A linear equation could best describe their relationship: Y(CFA-P)=0.927X(VMYC-P)-0.002. The Pearson's correlation coefficient was 0.985 with a significance level(n=69, P